Item type |
紀要論文(ELS) / Departmental Bulletin Paper(1) |
公開日 |
2012-12-01 |
タイトル |
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言語 |
ja |
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タイトル |
鉄存在下HepG2細胞におけるTNF-α/actinomycin D処理による肝障害モデルの構築 |
タイトル |
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言語 |
en |
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タイトル |
TNF-α/actinomycin D-mediated HepG2 cells in the presence of iron as a model of hepatocyte injury |
言語 |
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言語 |
jpn |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
tumor necrosis factor |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
cytotoxicity |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
actinomycin D |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
oxidative stress |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
iron |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
著者名 |
髙橋, 昌悟
三浦, 彩佳
佐々木, 瞳
坂口, 修平
永田, 清
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抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We examained the contribution of iron to the cytotoxicity of tumor necrosis factor (TNF)-a combined with actinomycin D (ActD) as a model of hepatocyte injury in Hep G2 cells. In general,hepatocytes are resistant to TNF-a. However,a transcriptional inhibitor such as ActD can sensitize then to TNF-a. In the present study, we show that low levels of ActD (0.5nM) sensitized HepG2 cells to the cytotoxic effects of TNF-a (20 ng/mL) for 48h. Iron plays a critical role in catalyxing the formation of potent oxidants. To acsses the toxicological significance of this TNF-a/ActD interaction,ferric-nitrilotriacetate (Fe-NTA,2uM) was added to the cells. Treatment with Fe-NTA significantly increased the sensitivity to the TNF-a/ActD-mediated cell death. TNF-a/ActD-mediated cell death in the presence of a lower concentration of iron did not result in DNA fragmentation. We suggest that iron increased the sensitivity to the cytotoxicity of TNF-a/ActD in HepG2 cells. It is likely that TNF-a/ActD/Fe-NTA-mediated cell death contributes to the non-apoptotic death of cells via oxidative stress caused by iron. Our experimental model may be useful for studying hepatic drug metabolism using TNF-a as a hepatocyte injury,especially in HepG2 cells. |
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言語 |
en |
書誌情報 |
ja : 東北薬科大学研究誌
en : Journal of Tohoku Pharmaceutical University
号 59,
p. 69-74,
発行日 2012-12
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雑誌書誌ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA1149311X |