| Item type |
紀要論文(ELS) / Departmental Bulletin Paper(1) |
| 公開日 |
2008-12-01 |
| タイトル |
|
|
タイトル |
RAW 264.7細胞におけるLPSで誘導される誘導型NO合成酵素の発現に対するCaffeic Acid Undecyl Esterの阻害について |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Inhibition of Lipopolysaccharide-induced Expression of Inducible Nitric Oxide Synthase by Caffeic Acid Undecyl Ester in RAW 264.7 Macrophages |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
caffeic acid undecyl ester;nitric oxide;inducible nitric oxide synthase;nuclear-factor-κB;RAW 264.7 macrophage |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者名 |
小山内, 優
菅野, 秀一
佐々木, 崇光
平塚, 真弘
石川, 正明
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We synthesized caffeic acid undecyl ester (CAUE),and found that it exhibited strong inhibitory effect of lipopolysaccharide (LPS) -induced nitric oxide (NO) production in murine macrophage-like RAW 264.7 cells.We examined the effect of CAUE on the expression inducible nitric oxide synthase (iNOS) in LPS-stimulated RAW 264.7 cells.Western blot analysis performed with specific anti-iNOS antibody showed that a decrease in NO was accompanied by a decrease in the level of iNOS protein with dose-dependent manner (CAUE:0.1～1.0μM).To clarify the mechanistic basis for CAUE's ability to inhibit the induction of iNOS,we examined the effect of CAUE on nuclear factor (NF)-κB,Inhibitor-κB (IκB) degradation and phosphorylation of extracellular-signaling regulated Kinases,ERK 1/2.CAUE potently suppressed the transcriptional activity of NF-κB,IκB degradation and activation of ERK.Since NF-κB was activated by following IκBα degradation,the recovery of IκBα protein indicating that CAUE inhibited the activation of degradation following NF-κB.These finding suggest that CAUE has the inhibitory effect on LPS-induced NO production and expression of iNOS in macrophage by the inhibition of IκB degradation and NF-κB activation,which may be mediated through blockage in the phospholylation ERK. |
|
言語 |
en |
| 書誌情報 |
ja : 東北薬科大学研究誌
en : Journal of Tohoku Pharmaceutical University
号 55,
p. 99-109,
発行日 2008-12
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| 雑誌書誌ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA1149311X |
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