{"_buckets": {"deposit": "b7052b65-68d8-460f-b5a8-1878a6c05b1b"}, "_deposit": {"created_by": 15, "id": "23", "owners": [15], "pid": {"revision_id": 0, "type": "depid", "value": "23"}, "status": "published"}, "_oai": {"id": "oai:tohoku-mpu.repo.nii.ac.jp:00000023", "sets": ["16"]}, "author_link": [], "item_1_biblio_info_14": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "59", "bibliographicPageEnd": "74", "bibliographicPageStart": "69", "bibliographic_titles": [{"bibliographic_title": "東北薬科大学研究誌", "bibliographic_titleLang": "ja"}, {"bibliographic_title": "Journal of Tohoku Pharmaceutical University", "bibliographic_titleLang": "en"}]}]}, "item_1_creator_6": {"attribute_name": "著者名(日)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "髙橋, 昌悟", "creatorNameLang": "ja"}, {"creatorName": "タカハシ, ショウゴ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Takahashi, Shogo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "三浦, 彩佳", "creatorNameLang": "ja"}, {"creatorName": "ミウラ, アヤカ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Miura, Ayaka", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "佐々木, 瞳", "creatorNameLang": "ja"}, {"creatorName": "ササキ, ヒトミ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Sasaki, Hitomi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "坂口, 修平", "creatorNameLang": "ja"}, {"creatorName": "サカグチ, シュウヘイ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Sakaguchi, Shuhei", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "永田, 清", "creatorNameLang": "ja"}, {"creatorName": "ナガタ, キヨシ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Nagata, Kiyoshi", "creatorNameLang": "en"}]}]}, "item_1_description_11": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "We examained the contribution of iron to the cytotoxicity of tumor necrosis factor (TNF)-a combined with actinomycin D (ActD) as a model of hepatocyte injury in Hep G2 cells. In general,hepatocytes are resistant to TNF-a. However,a transcriptional inhibitor such as ActD can sensitize then to TNF-a. In the present study, we show that low levels of ActD (0.5nM) sensitized HepG2 cells to the cytotoxic effects of TNF-a (20 ng/mL) for 48h. Iron plays a critical role in catalyxing the formation of potent oxidants. To acsses the toxicological significance of this TNF-a/ActD interaction,ferric-nitrilotriacetate (Fe-NTA,2uM) was added to the cells. Treatment with Fe-NTA significantly increased the sensitivity to the TNF-a/ActD-mediated cell death. TNF-a/ActD-mediated cell death in the presence of a lower concentration of iron did not result in DNA fragmentation. We suggest that iron increased the sensitivity to the cytotoxicity of TNF-a/ActD in HepG2 cells. It is likely that TNF-a/ActD/Fe-NTA-mediated cell death contributes to the non-apoptotic death of cells via oxidative stress caused by iron. Our experimental model may be useful for studying hepatic drug metabolism using TNF-a as a hepatocyte injury,especially in HepG2 cells.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_1_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA1149311X", "subitem_source_identifier_type": "NCID"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2012-12-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00008564959.pdf", "filesize": [{"value": "1.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1400000.0, "url": {"label": "KJ00008564959", "objectType": "fulltext", "url": "https://tohoku-mpu.repo.nii.ac.jp/record/23/files/KJ00008564959.pdf"}, "version_id": "210b7d59-70f0-46b6-b653-bac068e15b33"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "tumor necrosis factor", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cytotoxicity", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "actinomycin D", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "oxidative stress", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "iron", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "鉄存在下HepG2細胞におけるTNF-α/actinomycin D処理による肝障害モデルの構築", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "鉄存在下HepG2細胞におけるTNF-α/actinomycin D処理による肝障害モデルの構築", "subitem_title_language": "ja"}, {"subitem_title": "TNF-α/actinomycin D-mediated HepG2 cells in the presence of iron as a model of hepatocyte injury", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "15", "path": ["16"], "permalink_uri": "https://tohoku-mpu.repo.nii.ac.jp/records/23", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2012-12-01"}, "publish_date": "2012-12-01", "publish_status": "0", "recid": "23", "relation": {}, "relation_version_is_last": true, "title": ["鉄存在下HepG2細胞におけるTNF-α/actinomycin D処理による肝障害モデルの構築"], "weko_shared_id": -1}
鉄存在下HepG2細胞におけるTNF-α/actinomycin D処理による肝障害モデルの構築
https://tohoku-mpu.repo.nii.ac.jp/records/23
https://tohoku-mpu.repo.nii.ac.jp/records/23