| Item type |
紀要論文(ELS) / Departmental Bulletin Paper(1) |
| 公開日 |
2014-07-29 |
| タイトル |
|
|
タイトル |
ヒト癌細胞型ピルビン酸キナーゼ（PKM2）の過酸化水素に応答したレドックス状態変化の検出 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Detection of H2O2-induced changes in redox status of tumor-type pyruvate kinase（PKM2） |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
pyruvate kinase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
redox status |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cysteine oxidation |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者名 |
色川, 隼人
三好, 道世
菅原, 大輔
岩井, 健太
久下, 周佐
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Recent studies shed light on a crucial function of M2 isoform of mammalian pyruvate kinase（PkM2）on tumor cell growth. Pyruvate kinase activity of PkM2 was shown to be negatively regulated by growth factors or reactive oxygen species（ros）. The negative regulation of PkM2 enhances tumor cell growth. Despite biochemical and genetic evidences to indicate mechanism for the ros-induced negative regulation of PkM2, an apparent oxidized form of PkM2, which is induced by cellular ros such as H2o2, has not been identified so far. Thus, we aimed to identify oxidized PkM2 in cells in response to H2o2. To identify number of free cysteine thiols, we examined changes of molecular weight shift of PkM2, which is determined by the number of pegylated-maleimide molecule（Mw 2,000）bound to PkM2 thiols. our results suggested that one or two out of nine cysteine residues in PkM2 were oxidized in response to H2o2. our system may be useful to determine changes in PkM2 redox status in cells in response to ros-inducible environmental changes such as oxygen and nutrient availability. |
|
言語 |
en |
| 書誌情報 |
ja : 東北薬科大学研究誌
en : Journal of Tohoku Pharmaceutical University
号 60,
p. 49-54,
発行日 2013-12
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| 雑誌書誌ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA1149311X |
ヒト癌細胞型ピルビン酸キナーゼ（PKM2）の過酸化水素に応答したレドックス状態変化の検出 (725.6 kB)
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