| Item type |
紀要論文(ELS) / Departmental Bulletin Paper(1) |
| 公開日 |
2009-12-01 |
| タイトル |
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|
タイトル |
ラット肝ミトコンドリアMonoamine Oxidaseによる過酸化水素産生系に対する柴胡加龍骨牡蠣湯の影響 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Effect of Saiko-ka-ryoukotsu-borei-to on the Formation of Hydrogen Peroxide Generated by Rat Liver Mitochondria-monoamin Oxidase |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Saiko-ka-ryukotsu-borei-to ; monoamine oxidase ; hydrogen peroxidase ; ABTS/peroxidase colorimetric assay ; Scutellaria root ; flavonoids |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者名 |
佐々木, 健郎
伊東, 宗一
今野, 那美
吉﨑, 文彦
|
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Saiko-ka-ryukotsu-borei-to (SRBT) is adapted for psychoneurosis,such as autonomic dystonia and sleep disorder. Is has been postulated that reactive oxygen species (ROSs) plays an important role in psychoneurosis arising from oxidative neurogeneration.In this study,effect of SRBT on the formation of hydrogen peroxide generated by rat liver mitochondria oxdase (MAO) was investigated by enzymatic colorimetric assay using 2,2'-Azino-bis(3-ethyl benzthiazoline-6-sulfonic acid) (ABTS) oxidation catalyzed by peroxidase in vitro.SRBT (1mg/ml) had exhibited notable inhibition on the coupling reaction of ABTS radical cation with a hydrogen peroxide formed by peroxidase,suggesting inhibitory effect on monoamine oxdase activity.Comparative determination of the consequence on MAO with the lacking of each crude drug blended in SRBT indicated that Scutellaria root should by the active constituent participated with this activity.In Addition,when the MAO-inhibitory activity was compared with the partition of Sctellaria root decoction,the ethyl acetate-soluble layer had found to be the most effective in comparison with the other layers.Wogonin and baicalein had been revealed the principal components in ethyl acetate-soluble layer of Scutellaria root by TLC.These results have suggested that flavonoids derived from Scutellaria root might be involved in the inhibitory activity of SRBT on rat liver MAO. |
|
言語 |
en |
| 書誌情報 |
ja : 東北薬科大学研究誌
en : Journal of Tohoku Pharmaceutical University
号 56,
p. 55-58,
発行日 2009-12
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| 雑誌書誌ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA1149311X |
KJ00007286519 (894.5 kB)
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