| Item type |
紀要論文(ELS) / Departmental Bulletin Paper(1) |
| 公開日 |
2003-12-01 |
| タイトル |
|
|
タイトル |
In situ酵素組織化学法によるラット脳及び肝Monoamine Oxidase活性の検討 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
In Situ Quantitative Histochemical Analysis on Monoamine Oxidase Activity in Rat Brain and Liver |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
in situ histochemical analysis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
monoamine oxidase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
locus coeruleus |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
rat |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者名 |
佐々木, 健郎
伊東, 宗一
小瀧, 豊司
吉崎, 文彦
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
In situ quantitative histochemical analysis on monoamine oxidase (MAO) activity in rat brain and liver was performed for its region specificity. Nitro blue tetrazolium was used for the formazan producing reductive reaction caused by enzymatic degradation product of tryptamine as MAO substrate. A linear relationship was obserbed between the integrated MAO activity and the absorbance analyzed by PC-associated image analysis. The MAO-A inhibitor, clorgylin, inhibited the MAO activity dose-dependently in brain region and liver tissue in vitro and in situ analyses with well correlation. These results suggested that this method could be useful for the quantitative analysis of MAO activity in tissue sections, especially in the rat locus coeruleus that plays an important role in noradrenergic neurons. |
|
言語 |
en |
| 書誌情報 |
ja : 東北薬科大学研究誌
en : Journal of Tohoku Pharmaceutical University
号 50,
p. 125-130,
発行日 2003-12
|
| 雑誌書誌ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA1149311X |
KJ00004261039 (460.1 kB)
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