@article{oai:tohoku-mpu.repo.nii.ac.jp:00000788,
 author = {熊谷, 茉歩 and Kumagai, Maho and 石井, 敬 and Ishii, Makoto and 森本, かおり and Morimoto, Kaori and 富田, 幹雄 and Tomita, Mikio},
 issue = {66},
 journal = {東北医科薬科大学研究誌, Journal of Tohoku Medical and Pharmaceutical University},
 month = {Dec},
 note = {We investigated the expression levels of major ATP-binding cassette transporters such as P-glycoprotein（P-gp）and CYP metabolic enzymes in the liver and small and large intestines during ulcerative colitis（UC）．In addition, we examined the involvement of tight junctions（TJ）such as intestinal epithelial cells with barrier functions by measuring membrane resistance（Rm），relative activity of P-gp evaluated by a P-gp substrate, and rhodamine 123（Rho123）permeability in the presence or absence of a specific P-gp inhibitor, verapamil. Expression levels of both P-gp and CYP decreased in the liver, suggesting a secondary loss of barrier function in UC. A decreasein P-gp and an increase in CYP was observed in the jejunum and colon, but not in the ileum. In control conditions,the permeability of Rho123 in the serosal to mucosal direction was greater than that in the reverse direction, as  observed in an in vitro diffusion chamber method. The inhibition of Rho123 permeability was observed in the presence of verapamil, indicating P-gp was sufficiently expressed and was working fine in the small and large intestines. In contrast, a decrease in the relative activity of P-gp and Rm, and Rho123 hyperpermeability in both directions was observed in UC, when compared with control conditions. These data indicate that hyper-permeability of Rho123 in the small intestine resulted from not only P-gp dysfunction, but also the decrease in Rm. The plasma  concentration profile of Rho123 after intravenous and oral administrations showed that UC affected Rho123 absorption from the small intestine and liver functions, as evaluated by Rho123 elimination.},
 pages = {45--54},
 title = {デキストラン硫酸ナトリウム誘発性潰瘍性大腸炎ラットにおける肝および腸バリア機能の変動},
 year = {2019},
 yomi = {クマガイ, マホ and イシイ, マコト and モリモト, カオリ and トミタ, ミキオ}
}