{"created":"2023-06-20T13:11:33.289321+00:00","id":767,"links":{},"metadata":{"_buckets":{"deposit":"8cc91e7a-9806-48d5-83ca-68386e07ee90"},"_deposit":{"created_by":15,"id":"767","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"767"},"status":"published"},"_oai":{"id":"oai:tohoku-mpu.repo.nii.ac.jp:00000767","sets":["3:70"]},"author_link":[],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"65","bibliographicPageEnd":"28","bibliographicPageStart":"21","bibliographic_titles":[{"bibliographic_title":"東北医科薬科大学研究誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Journal of Tohoku Medical and Pharmaceutical University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"globotriaosylceramide（gb3）is the most important cell surface ligand of the rhamnose-binding lectins such as Sirulus asotus lectin（SaL）．SaL recognizes and binds to gb3 expressing tumor cells such as Burkitts lymphoma raji cells and induces them to the g0/g1 phase growth arrest. Thus, a fine detecting probe, or monoclonal antibody （mab）of gb3, is important for operating immunocytochemical analyses of SaL. in this experiments, we established simple and convenience preparation method to obtain the mab, 1a4（isotype: igM）, from the culture medium of the provided hybridoma cell line（originally from ishigami F., and hakomori S., method unpublished）, and compared its reactivity and stability with Bgr23（isotype: igg）which is commonly used and commercially available mab against gb3. mab 1a4 was simply purified by using rapid SPin L column within an hour and showed higher reactivity than Bgr23 on the flow cytometric analyses against gb3 expressing tumor cells. The optimum concentration of 1a4 was 0.6μg/mL for the adequate detection while four and ten times higher concentrations were needed to obtain equal reactivity on raji and ToS-1 cells, respectively, by using Bgr23. although purified 1a4 was stable in the 0.1 M Tris-glycine after one-month storage at 4℃ or −80℃, its reactivity was slightly decreased when the buffer was changed to 10 mM PBS. These results suggest that mab 1a4 possesses relatively higher binding capacity to cell surface gb3 than Bgr23 and highly concentrated buffer could also suitable for its storage condition.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東北医科薬科大学","subitem_publisher_language":"ja"}]},"item_10002_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0)","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by-nc-nd/3.0/deed.en"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2432-5724","subitem_source_identifier_type":"PISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"菅原, 栄紀","creatorNameLang":"ja"},{"creatorName":"スガラワ, シゲキ","creatorNameLang":"ja-Kana"},{"creatorName":"Sugawara, Shigeki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"鷹觜, 祥子","creatorNameLang":"ja"},{"creatorName":"タカノハシ, ショウコ","creatorNameLang":"ja-Kana"},{"creatorName":"Takanohashi, Shoko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"本田, 捷太","creatorNameLang":"ja"},{"creatorName":"ホンダ, ショウタ","creatorNameLang":"ja-Kana"},{"creatorName":"Honda, Shota","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"立田, 岳生","creatorNameLang":"ja"},{"creatorName":"タツタ, タケオ","creatorNameLang":"ja-Kana"},{"creatorName":"Tatsuta, Takeo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"伊藤, 淳","creatorNameLang":"ja"},{"creatorName":"イトウ, ジュン","creatorNameLang":"ja-Kana"},{"creatorName":"Ito, Jun","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"佐藤, 信","creatorNameLang":"ja"},{"creatorName":"サトウ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"Sato, Makoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"大山, 力","creatorNameLang":"ja"},{"creatorName":"オオヤマ, チカラ","creatorNameLang":"ja-Kana"},{"creatorName":"Ohyama, Chikara","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"細野, 雅祐","creatorNameLang":"ja"},{"creatorName":"ホソノ, マサヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Hosono, Masahiro","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-01-15"}],"displaytype":"detail","filename":"原著1_菅原栄紀.pdf","filesize":[{"value":"3.1 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"Gb3 検出に利用する抗Gb3 抗体（1A4）の有用性の検討","objectType":"fulltext","url":"https://tohoku-mpu.repo.nii.ac.jp/record/767/files/原著1_菅原栄紀.pdf"},"version_id":"b307a1fc-3f88-481c-b3c7-a08f6137c3d6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"globotriaosylceramide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"monoclonal antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"flowcytometory","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Gb3 検出に利用する抗Gb3 抗体（1A4）の有用性の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Gb3 検出に利用する抗Gb3 抗体（1A4）の有用性の検討","subitem_title_language":"ja"},{"subitem_title":"Examination of the Usability of Anti-Gb3 Antibody（1A4）for Detection of Gb3","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"15","path":["70"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-01-15"},"publish_date":"2020-01-15","publish_status":"0","recid":"767","relation_version_is_last":true,"title":["Gb3 検出に利用する抗Gb3 抗体（1A4）の有用性の検討"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-03-03T23:41:29.464508+00:00"}