@article{oai:tohoku-mpu.repo.nii.ac.jp:00000767,
 author = {菅原, 栄紀 and Sugawara, Shigeki and 鷹觜, 祥子 and Takanohashi, Shoko and 本田, 捷太 and Honda, Shota and 立田, 岳生 and Tatsuta, Takeo and 伊藤, 淳 and Ito, Jun and 佐藤, 信 and Sato, Makoto and 大山, 力 and Ohyama, Chikara and 細野, 雅祐 and Hosono, Masahiro},
 issue = {65},
 journal = {東北医科薬科大学研究誌, Journal of Tohoku Medical and Pharmaceutical University},
 month = {Dec},
 note = {globotriaosylceramide（gb3）is the most important cell surface ligand of the rhamnose-binding lectins such as Sirulus asotus lectin（SaL）．SaL recognizes and binds to gb3 expressing tumor cells such as Burkitts lymphoma raji cells and induces them to the g0/g1 phase growth arrest. Thus, a fine detecting probe, or monoclonal antibody （mab）of gb3, is important for operating immunocytochemical analyses of SaL. in this experiments, we established simple and convenience preparation method to obtain the mab, 1a4（isotype: igM）, from the culture medium of the provided hybridoma cell line（originally from ishigami F., and hakomori S., method unpublished）, and compared its reactivity and stability with Bgr23（isotype: igg）which is commonly used and commercially available mab against gb3. mab 1a4 was simply purified by using rapid SPin L column within an hour and showed higher reactivity than Bgr23 on the flow cytometric analyses against gb3 expressing tumor cells. The optimum concentration of 1a4 was 0.6μg/mL for the adequate detection while four and ten times higher concentrations were needed to obtain equal reactivity on raji and ToS-1 cells, respectively, by using Bgr23. although purified 1a4 was stable in the 0.1 M Tris-glycine after one-month storage at 4℃ or −80℃, its reactivity was slightly decreased when the buffer was changed to 10 mM PBS. These results suggest that mab 1a4 possesses relatively higher binding capacity to cell surface gb3 than Bgr23 and highly concentrated buffer could also suitable for its storage condition.},
 pages = {21--28},
 title = {Gb3 検出に利用する抗Gb3 抗体（1A4）の有用性の検討},
 year = {2018},
 yomi = {スガラワ, シゲキ and タカノハシ, ショウコ and ホンダ, ショウタ and タツタ, タケオ and イトウ, ジュン and サトウ, マコト and オオヤマ, チカラ and ホソノ, マサヒロ}
}