@article{oai:tohoku-mpu.repo.nii.ac.jp:00000729,
 author = {内田, 龍児 and Uchida, Ryuji},
 issue = {64},
 journal = {東北医科薬科大学研究誌, Journal of Tohoku Medical and Pharmaceutical University},
 month = {Dec},
 note = {In the process of new antibiotic developments, many drug candidates from in vitro assay systems often have no therapeutic effects in in vivo animal models using mammal.
To overcome this problem, we established an in vivomimic infection assay using silkworm larvae as a host animal, and started to apply this assay to the primary screening for new antibiotics of microbial origin.
Comparing with an in vitro assay using paper disk method, the hit rate of candidate culture broths showing a therapeutic effect in silkworm infection assay markedly decreased, but isolated compounds have included some clinical drugs and their related compounds.
Furthermore, we discovered new anti-MRSa antibiotics, designated nosokomycins A to D, from the culture broth of Streptomyces cyslabdanicas K04-0144, and demonstrated that nosokomycin A also showed a good therapeutic effect in an in vivo mouse infection assay as well as silkworm infection assay.
Thus, we predicted that candidate compounds discovered by silkworm infection assay would have higher potential as antibiotic leads.},
 pages = {1--16},
 title = {微生物資源からの新規抗生物質のスクリーニング－簡易的なin vivo様評価系: カイコ感染症モデルを利用して－},
 year = {2017},
 yomi = {ウチダ, リュウジ}
}